METHODS: Patients with SLE were matched by age, sex and ethnic background to healthcare worker healthy controls (HC)
Anti-SARS-CoV-2 spike glycoprotein antibodies were measured at 4-8 weeks following the second COVID-19 vaccine dose (either BNT162b2 or ChAdOx1 nCoV-19) using a CE-marked combined ELISA detecting IgG, IgA and IgM (IgGAM). Antibody levels were considered as a continuous variable and in tertiles and compared between SLE patients and HC and associations with medication, disease activity and serological parameters were determined. RESULTS: Antibody levels were lower in 43 SLE patients compared to 40 HC (p < 0.001). There was no association between antibody levels and medication, lupus disease activity, vaccine type or prior COVID infection. Higher serum IgA, but not IgG or IgM, was associated with being in a higher anti-SARS-CoV-2 antibody level tertile (OR [95% CI] 1.
820 [1.050, 3.156] p = 0.033). Similarly, higher lymphocyte count was also associated with being in a higher tertile of anti-SARS-CoV-2 (OR 3.330 [1.505, 7.
366] p = 0.003). vitamin d3 benefits : Patients with SLE have lower antibody levels following 2 doses of COVID-19 vaccines compared to HC. In SLE lower lymphocyte counts and serum IgA levels are associated with lower antibody levels post vaccination, potentially identifying a subgroup of patients who may therefore be to the research, authorship, and/or publication of this article: Caroline Gordon reports personal fees for honoraria from consultancy work and/or advisory boards from the Center for Disease Control and Prevention, AbbVie, Amgen, Astra-Zeneca, BMS, GSK, MGP, Sanofi, UCB; personal fees for speakers bureau from UCB and GSK; previous educational grants from UCB to Sandwell and West Birmingham Hospitals NHS Trust that have supported her research work. Sofia Tosounidou reports O somatic antigens have been studied in young fistulated pigs. Antibody levels in intestinal secretion were raised for approximately 2–3 weeks following a single local antigenic challenge with a heat-killed aqueous suspension of E. coli.
A second challenge provoked a similar response suggesting a lack of immunological memory. Antibody activity in the secretions was predominantly associated with IgA and immunofluorescent studies of biopsy specimens from young fistulated animals indicated that intestinal synthesis and secretion of IgA had commenced by the 10th day of life. Studies of piglets reared with the sow indicated that oral immunization with E. coli antigen after 10 days of age stimulated intestinal antibody secretion before weaning at 3 weeks. The response of gnotobiotic pigs to oral immunization and infection was evaluated by immunofluorescent histology of the intestinal mucosa. Repeated oral administration of heat-killed E. coli O8 resulted in an immunocyte response in the lamina propria numerically comparable with that produced by infection with the live organism.
The early response was dominated by cells of the IgM class whereas after 3 weeks IgA cells predominated. In d3 vitamin -free animal very few immunoglobulin-containing cells were detected. In vitro studies of antibacterial activity indicated that the most probable mechanism of immunological control in the alimentary tract is bacteriostasis.Investigation of Live and Inactivated Vaccines.common in endemic areas of Germany in the 20th century but was abandoned in the early 1990s. The recent occurrence of fatal cases of human encephalitis due to Borna disease virus 1 (BoDV-1) has rekindled the interest in vaccination. (2) Methods: The full genomes of the BD live vaccine viruses "Dessau" and "Giessen" were sequenced and analyzed for the first time.
All vaccination experiments followed a proof-of-concept approach. Dose-titration infection experiments were performed in rabbits, based on both cell culture- and brain-derived viruses at various doses. Inactivated vaccines against BD were produced from concentrated cell culture supernatants and investigated in rabbits and horses. The BoDV-1 live vaccine "Dessau" was administered to horses and antibody profiles were determined. (3) Results: The BD live vaccine viruses "Dessau" and "Giessen" belong to clusters 3 and 4 of BoDV-1. Whereas the "Giessen" virus does not differ substantially from field viruses, the "Dessau" virus shows striking differences in the M gene and the N-terminal part of the G gene.